PGR News from the Pacific

Posted 4:52 PM by Luigi

Taro paper

Evaluation of variability among breeding lines and cultivars of taro (Colocasia esculenta) in Papua New Guinea using ISSR fingerprinting and agro-morphological characterization

Agro-morphological characterization and inter-simple sequence repeat (ISSR) DNA fingerprinting were used to evaluate variability among selected Papua New Guinean (PNG) taro (Colocasia esculenta) accessions. Twenty-three discriminatory descriptors selected from the International Plant Genetic Resources Institute (IPGRI, 1999) descriptor list were combined with ISSR markers to characterize 13 taro accessions. Genotyping data were obtained via polymerase chain reaction (PCR) with three ISSR primers: (GA)9AT, (GA)9AC and (ACC)6Y. Initial evaluation of the agro-morphological variation indicated that the total variation observed appeared to be associated with colours of corm flesh, corm cortex and petiole, stolon formation, corm shape, lamina orientation and lamina vein pattern. All genotypes were separated as distinct morphotypes, providing useful information for development of a minimum descriptor list. ISSR primers generated complex banding patterns, with primer (GA)9AC amplifying fewer but proportionately more (90%) polymorphic fragments. Fingerprinting with three ISSR primers enabled the identification of closely related genotypes and the separation of those that were distantly related. Little correlation was found in between the agro-morphological and molecular data in the clusters generated. However, the two types of data provided complementary information on the existing diversity and available genotypes that can be immediately used by the breeders.

By T. Okpul E.S. Mace I.D. Godwin D. Singh M.E. Wagih

Article published in Plant Genetic Resources Newsletter No.143, 2005 in English Page 8 to 16

[Read more...]



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DIVA-GIS	Monday, February 13, 2006 Posted 4:52 PM by Luigi Taro paper *Evaluation of variability among breeding lines and cultivars of taro (Colocasia esculenta) in Papua New Guinea using ISSR fingerprinting and agro-morphological characterization* Agro-morphological characterization and inter-simple sequence repeat (ISSR) DNA fingerprinting were used to evaluate variability among selected Papua New Guinean (PNG) taro (Colocasia esculenta) accessions. Twenty-three discriminatory descriptors selected from the International Plant Genetic Resources Institute (IPGRI, 1999) descriptor list were combined with ISSR markers to characterize 13 taro accessions. Genotyping data were obtained via polymerase chain reaction (PCR) with three ISSR primers: (GA)9AT, (GA)9AC and (ACC)6Y. Initial evaluation of the agro-morphological variation indicated that the total variation observed appeared to be associated with colours of corm flesh, corm cortex and petiole, stolon formation, corm shape, lamina orientation and lamina vein pattern. All genotypes were separated as distinct morphotypes, providing useful information for development of a minimum descriptor list. ISSR primers generated complex banding patterns, with primer (GA)9AC amplifying fewer but proportionately more (90%) polymorphic fragments. Fingerprinting with three ISSR primers enabled the identification of closely related genotypes and the separation of those that were distantly related. Little correlation was found in between the agro-morphological and molecular data in the clusters generated. However, the two types of data provided complementary information on the existing diversity and available genotypes that can be immediately used by the breeders. By T. Okpul E.S. Mace I.D. Godwin D. Singh M.E. Wagih Article published in Plant Genetic Resources Newsletter No.143, 2005 in English Page 8 to 16 [Read more...] * Comments: Post a Comment	Archive October 2002 November 2002 December 2002 January 2003 February 2003 March 2003 April 2003 May 2003 June 2003 July 2003 August 2003 September 2003 October 2003 November 2003 December 2003 January 2004 February 2004 March 2004 April 2004 May 2004 June 2004 July 2004 August 2004 September 2004 October 2004 November 2004 December 2004 January 2005 February 2005 March 2005 April 2005 May 2005 June 2005 July 2005 August 2005 September 2005 October 2005 November 2005 December 2005 January 2006 February 2006 March 2006 April 2006 May 2006 June 2006 July 2006 August 2006 September 2006 October 2006 November 2006 December 2006 January 2007 February 2007 March 2007 April 2007 May 2007 June 2007 July 2007 August 2007 September 2007 October 2007 November 2007 December 2007 January 2008 February 2008 March 2008 April 2008 May 2008 June 2008 July 2008 August 2008 September 2008 October 2008 November 2008 December 2008 January 2009 February 2009 March 2009 April 2009 May 2009 June 2009 July 2009 August 2009 September 2009 October 2009 November 2009 January 2010 RSS Feed Alternative feed Contact Tevita Something new: Agrobiodiversity Weblog: For discussions of conservation and sustainable use of the genetic resources of crops, livestock and their wild relatives. 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