A blog maintained by Tevita Kete, PGR Officer
Secretariat of the Pacific Community (SPC), Suva, Fiji Islands
This weblog documents the activities of Pacific Agricultural Genetic Resources Network (PAPGREN), along with other information on plant genetic resources (PGR) in the Pacific.
The myriad varieties found within cultivated plants are fundamental to the present and future productivity of agriculture. PAPGREN, which is coordinated by the Land Resources Division of the Secretariat of the Pacific Community (SPC), helps Pacific countries and territories to conserve their crop genetic diversity sustainably, with technical assistance from the Bioversity International (BI) and support from NZAID and ACIAR.
SPC also hosts the Centre of Pacific Crops and Trees (CEPaCT). The CEPaCT maintains regional in vitro collections of crops important to the Pacific and carries out research on tissue culture technology. The CEPaCT Adviser is Dr Mary Taylor (MaryT@spc.int), the CEPaCT Curator is Ms Valerie Tuia (ValerieT@spc.int).
PAPGREN coordination and support
Mr William Wigmore
Mr Adelino S. Lorens
Dr Lois Englberger
Mr Apisai Ucuboi
Dr Maurice Wong
Mr Tianeti Beenna Ioane
Mr Frederick Muller
Mr Herman Francisco
Ms Rosa Kambuou
Ms Laisene Samuelu
Mr Jimi Saelea
Mr Tony Jansen
Mr Finao Pole
Mr Frazer Bule Lehi
Interested in GIS?
Monday, February 13, 2006
Posted 4:52 PM by Luigi
Evaluation of variability among breeding lines and cultivars of taro (Colocasia esculenta) in Papua New Guinea using ISSR fingerprinting and agro-morphological characterization
Agro-morphological characterization and inter-simple sequence repeat (ISSR) DNA fingerprinting were used to evaluate variability among selected Papua New Guinean (PNG) taro (Colocasia esculenta) accessions. Twenty-three discriminatory descriptors selected from the International Plant Genetic Resources Institute (IPGRI, 1999) descriptor list were combined with ISSR markers to characterize 13 taro accessions. Genotyping data were obtained via polymerase chain reaction (PCR) with three ISSR primers: (GA)9AT, (GA)9AC and (ACC)6Y. Initial evaluation of the agro-morphological variation indicated that the total variation observed appeared to be associated with colours of corm flesh, corm cortex and petiole, stolon formation, corm shape, lamina orientation and lamina vein pattern. All genotypes were separated as distinct morphotypes, providing useful information for development of a minimum descriptor list. ISSR primers generated complex banding patterns, with primer (GA)9AC amplifying fewer but proportionately more (90%) polymorphic fragments. Fingerprinting with three ISSR primers enabled the identification of closely related genotypes and the separation of those that were distantly related. Little correlation was found in between the agro-morphological and molecular data in the clusters generated. However, the two types of data provided complementary information on the existing diversity and available genotypes that can be immediately used by the breeders.
By T. Okpul E.S. Mace I.D. Godwin D. Singh M.E. Wagih
Article published in Plant Genetic Resources Newsletter No.143, 2005 in English Page 8 to 16
* Comments:Post a Comment
Agrobiodiversity Weblog: For discussions of conservation and sustainable use of the genetic resources of crops, livestock and their wild relatives.