PGR News from the Pacific

Posted 2:14 PM by Luigi

New in vitro conservation paper from RGC

CRYOPRESERVATION OF IN VITRO-GROWN SHOOT-TIPS OF TROPICAL TARO (COLOCASIA ESCULENTA VAR. ESCULENTA) BY VITRIFICATION

Rajnesh Sant, Mary Taylor, Anand Tyagi

Abstract

In vitro shoot-tips of three cultivars of tropical taro (Colocasia esculenta var. esculenta (L.) Schott) were successfully cryopreserved by vitrification. Different conditioning treatments were required for each of the cultivars, while the vitrification protocol was constant for all. For the cultivars E399 and CPUK, shoot-tips from three-month-old in vitro plants grown on solidified MS were preconditioned on MS with 0.3 M sucrose in the dark for 16 h at 25oC. For the cultivar TNS, donor plants were preconditioned on solid MS with 90 gL-1 sucrose for seven weeks before cryopreservation. For vitrification, the shoot-tips were loaded with a solution of 2 M glycerol plus 0.4 M sucrose for 20 min at 25oC, dehydrated with PVS2 for 12 min at 25oC and plunged in liquid nitrogen. Vials were warmed by rapid shaking in a water bath at 40oC for 1 min 30 sec. Shoot-tips were rehydrated in liquid MS with 1.2 M sucrose for 15 min at 25oC then plated on recovery medium. Shoot-tips resumed growth within a week and developed into plantlets six to eight weeks later without any callus formation. Best percentage mean recoveries for the three cultivars were 21, 29 and 30% for E399, CPUK and TNS, respectively. This protocol was evaluated with five other taro cultivars with no success. However, this study has shown that vitrification has potential for cryopreserving tropical taro.

Keywords: shoot-tips, cryopreservation, vitrification, tropical taro (Colocasia esculenta var. esculenta (L.) Schott), preculture, preconditioning



	A blog maintained by Tevita Kete, PGR Officer Secretariat of the Pacific Community (SPC), Suva, Fiji Islands
This weblog documents the activities of Pacific Agricultural Genetic Resources Network (PAPGREN), along with other information on plant genetic resources (PGR) in the Pacific. The myriad varieties found within cultivated plants are fundamental to the present and future productivity of agriculture. PAPGREN, which is coordinated by the Land Resources Division of the Secretariat of the Pacific Community (SPC), helps Pacific countries and territories to conserve their crop genetic diversity sustainably, with technical assistance from the Bioversity International (BI) and support from NZAID and ACIAR. SPC also hosts the Centre of Pacific Crops and Trees (CEPaCT). The CEPaCT maintains regional in vitro collections of crops important to the Pacific and carries out research on tissue culture technology. The CEPaCT Adviser is Dr Mary Taylor (MaryT@spc.int), the CEPaCT Curator is Ms Valerie Tuia (ValerieT@spc.int). 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DIVA-GIS	Monday, May 29, 2006 Posted 2:14 PM by Luigi *New in vitro* conservation paper from RGC** CRYOPRESERVATION OF IN VITRO-GROWN SHOOT-TIPS OF TROPICAL TARO (COLOCASIA ESCULENTA VAR. ESCULENTA) BY VITRIFICATION Rajnesh Sant, Mary Taylor, Anand Tyagi Abstract In vitro shoot-tips of three cultivars of tropical taro (Colocasia esculenta var. esculenta (L.) Schott) were successfully cryopreserved by vitrification. Different conditioning treatments were required for each of the cultivars, while the vitrification protocol was constant for all. For the cultivars E399 and CPUK, shoot-tips from three-month-old in vitro plants grown on solidified MS were preconditioned on MS with 0.3 M sucrose in the dark for 16 h at 25oC. For the cultivar TNS, donor plants were preconditioned on solid MS with 90 gL-1 sucrose for seven weeks before cryopreservation. For vitrification, the shoot-tips were loaded with a solution of 2 M glycerol plus 0.4 M sucrose for 20 min at 25oC, dehydrated with PVS2 for 12 min at 25oC and plunged in liquid nitrogen. Vials were warmed by rapid shaking in a water bath at 40oC for 1 min 30 sec. Shoot-tips were rehydrated in liquid MS with 1.2 M sucrose for 15 min at 25oC then plated on recovery medium. Shoot-tips resumed growth within a week and developed into plantlets six to eight weeks later without any callus formation. Best percentage mean recoveries for the three cultivars were 21, 29 and 30% for E399, CPUK and TNS, respectively. This protocol was evaluated with five other taro cultivars with no success. However, this study has shown that vitrification has potential for cryopreserving tropical taro. Keywords: shoot-tips, cryopreservation, vitrification, tropical taro (Colocasia esculenta var. esculenta (L.) Schott), preculture, preconditioning * Comments: Post a Comment	Archive October 2002 November 2002 December 2002 January 2003 February 2003 March 2003 April 2003 May 2003 June 2003 July 2003 August 2003 September 2003 October 2003 November 2003 December 2003 January 2004 February 2004 March 2004 April 2004 May 2004 June 2004 July 2004 August 2004 September 2004 October 2004 November 2004 December 2004 January 2005 February 2005 March 2005 April 2005 May 2005 June 2005 July 2005 August 2005 September 2005 October 2005 November 2005 December 2005 January 2006 February 2006 March 2006 April 2006 May 2006 June 2006 July 2006 August 2006 September 2006 October 2006 November 2006 December 2006 January 2007 February 2007 March 2007 April 2007 May 2007 June 2007 July 2007 August 2007 September 2007 October 2007 November 2007 December 2007 January 2008 February 2008 March 2008 April 2008 May 2008 June 2008 July 2008 August 2008 September 2008 October 2008 November 2008 December 2008 January 2009 February 2009 March 2009 April 2009 May 2009 June 2009 July 2009 August 2009 September 2009 October 2009 November 2009 January 2010 RSS Feed Alternative feed Contact Tevita Something new: Agrobiodiversity Weblog: For discussions of conservation and sustainable use of the genetic resources of crops, livestock and their wild relatives. 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