A blog maintained by Tevita Kete, PGR Officer
Secretariat of the Pacific Community (SPC), Suva, Fiji Islands
This weblog documents the activities of Pacific Agricultural Genetic Resources Network (PAPGREN), along with other information on plant genetic resources (PGR) in the Pacific.
The myriad varieties found within cultivated plants are fundamental to the present and future productivity of agriculture. PAPGREN, which is coordinated by the Land Resources Division of the Secretariat of the Pacific Community (SPC), helps Pacific countries and territories to conserve their crop genetic diversity sustainably, with technical assistance from the Bioversity International (BI) and support from NZAID and ACIAR.
SPC also hosts the Centre of Pacific Crops and Trees (CEPaCT). The CEPaCT maintains regional in vitro collections of crops important to the Pacific and carries out research on tissue culture technology. The CEPaCT Adviser is Dr Mary Taylor (MaryT@spc.int), the CEPaCT Curator is Ms Valerie Tuia (ValerieT@spc.int).
PAPGREN coordination and support
Mr William Wigmore
Mr Adelino S. Lorens
Dr Lois Englberger
Mr Apisai Ucuboi
Dr Maurice Wong
Mr Tianeti Beenna Ioane
Mr Frederick Muller
Mr Herman Francisco
Ms Rosa Kambuou
Ms Laisene Samuelu
Mr Jimi Saelea
Mr Tony Jansen
Mr Finao Pole
Mr Frazer Bule Lehi
Interested in GIS?
Wednesday, May 07, 2008
Posted 3:06 PM by Tevita
Fighting Fusarium in Asia-Pacific
- ACIAR project update
From : RISBAP
The ACIAR-funded project, called “Mitigating the threat of banana Fusarium
wilt: Understanding the agro-ecological distribution of pathogenic forms and
developing disease management strategies”, continues to progress as it conducts survey and collection in Papua New Guinea, VCG analysis and molecular characterization, virulence-host resistance study, and establishment of on-farm demo-trials of disease management strategies. Survey and Collection in PNG. To date, survey and collection activities are being conducted in PNG. Survey teams included Jeff Daniells (Taxonomist), Pere Kokoa (Plant Pathologist), Birte Komolong (Plant Pathologist) and Janet Pafoa (Taxonomist). Survey was carried out between August to September 2007. A total of 27 samples from plants showing symptoms suspected to be of
Fusarium wilt were collected from different provinces : Morobe Province/ Markham Valley, Kainantu, North Solomon Province/ Buka and ENB. The limited samples collected reflected the very low level of plants showing “Fusarium wilt symptoms”. No fusarium wilt symptoms were observed in ENB province. These samples were processed and then sent to QDPI, Australia for pathogen verification and VCG analyses. Other remaining provinces were to be surveyed late last year but was postponed to first quarter of 2008. These include Sandaun Province, Western Province and Manus Province, important provinces bordering Indonesia. These provinces were identified based on previous reports of suspicious Fusarium wilt symptoms in some areas. VCG analysis and Molecular Characterization. A total of 111 pure
isolate-Foc samples from the Indonesian Tropical Fruits Research Institute
(ITFRURI) were sent to QDPI for VCG and DNA characterization. To date, 80 of
the 111 Indonesian isolates have been VCG tested. The rest have been analysed
for volatile production and have produced mutant sectors. These samples are
now ready for VCG testing. Based on the samples tested from different Indonesian provinces, majority of the positive results have been identified to belong to VCG 01213/16 (Tropical Race 4 – TR4). There are also positive results
for other VCGs in Indonesian provinces and potentially new VCG record for
Indonesia. The new records will be re-tested and re-confirmed, thus expanding
the knowledge of the nature and distribution of Foc VCGs in Indonesia.
A total of 27 samples from PNG for VCG testing were received by QDPI. No Foc pathogen was isolated from the 1st batch of 16 samples received from PNG. The 2nd batch of 11 samples are currently being tested. Virulence-host resistance study. A total of 25 varieties were selected for inoculation with various VCGs for virulence/host resistance test. Preliminary inoculation trial was done on 4 varieties namely: Ambon Kuning(AAA), Barangan (AAA), Kepok (ABB), and Rajasere (AAB). This is a screenhouse test that involves inoculating tissue culture plantlets, with selected VCGs. Then external as well as internal symptoms are quantified and recorded. A more complete inoculation studies on the 25 selected varieties are being established. On-farm disease management demo-trials of disease management strategies. A participatory planning workshop was conducted in the two sites on November 26 – 30, 2007. During the workshop, details of the methodology,
field set up, and control practices to be evaluated were discussed and agreed
upon. Planting materials were being prepared by ITFRURI. The establishment
of the the demonstration plots was scheduled between January to February
2008. Survey and listing of existing private laboratories that produce banana
tissue culture are being conducted in preparation for the implementation of
the banana tissue cultured meriplant delivery system in Indonesia. (For
more information contact Dr. A. Molina - Project Coordinator at email@example.com)
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