PGR News from the Pacific

Posted 3:06 PM by Tevita

Fighting Fusarium in Asia-Pacific
- ACIAR project update

From : RISBAP

The ACIAR-funded project, called “Mitigating the threat of banana Fusarium
wilt: Understanding the agro-ecological distribution of pathogenic forms and
developing disease management strategies”, continues to progress as it conducts survey and collection in Papua New Guinea, VCG analysis and molecular characterization, virulence-host resistance study, and establishment of on-farm demo-trials of disease management strategies. Survey and Collection in PNG. To date, survey and collection activities are being conducted in PNG. Survey teams included Jeff Daniells (Taxonomist), Pere Kokoa (Plant Pathologist), Birte Komolong (Plant Pathologist) and Janet Pafoa (Taxonomist). Survey was carried out between August to September 2007. A total of 27 samples from plants showing symptoms suspected to be of
Fusarium wilt were collected from different provinces : Morobe Province/ Markham Valley, Kainantu, North Solomon Province/ Buka and ENB. The limited samples collected reflected the very low level of plants showing “Fusarium wilt symptoms”. No fusarium wilt symptoms were observed in ENB province. These samples were processed and then sent to QDPI, Australia for pathogen verification and VCG analyses. Other remaining provinces were to be surveyed late last year but was postponed to first quarter of 2008. These include Sandaun Province, Western Province and Manus Province, important provinces bordering Indonesia. These provinces were identified based on previous reports of suspicious Fusarium wilt symptoms in some areas. VCG analysis and Molecular Characterization. A total of 111 pure
isolate-Foc samples from the Indonesian Tropical Fruits Research Institute
(ITFRURI) were sent to QDPI for VCG and DNA characterization. To date, 80 of
the 111 Indonesian isolates have been VCG tested. The rest have been analysed
for volatile production and have produced mutant sectors. These samples are
now ready for VCG testing. Based on the samples tested from different Indonesian provinces, majority of the positive results have been identified to belong to VCG 01213/16 (Tropical Race 4 – TR4). There are also positive results
for other VCGs in Indonesian provinces and potentially new VCG record for
Indonesia. The new records will be re-tested and re-confirmed, thus expanding
the knowledge of the nature and distribution of Foc VCGs in Indonesia.
A total of 27 samples from PNG for VCG testing were received by QDPI. No Foc pathogen was isolated from the 1st batch of 16 samples received from PNG. The 2nd batch of 11 samples are currently being tested. Virulence-host resistance study. A total of 25 varieties were selected for inoculation with various VCGs for virulence/host resistance test. Preliminary inoculation trial was done on 4 varieties namely: Ambon Kuning(AAA), Barangan (AAA), Kepok (ABB), and Rajasere (AAB). This is a screenhouse test that involves inoculating tissue culture plantlets, with selected VCGs. Then external as well as internal symptoms are quantified and recorded. A more complete inoculation studies on the 25 selected varieties are being established. On-farm disease management demo-trials of disease management strategies. A participatory planning workshop was conducted in the two sites on November 26 – 30, 2007. During the workshop, details of the methodology,
field set up, and control practices to be evaluated were discussed and agreed
upon. Planting materials were being prepared by ITFRURI. The establishment
of the the demonstration plots was scheduled between January to February
2008. Survey and listing of existing private laboratories that produce banana
tissue culture are being conducted in preparation for the implementation of
the banana tissue cultured meriplant delivery system in Indonesia. (For
more information contact Dr. A. Molina - Project Coordinator at a.molina@cgiar.org)



	A blog maintained by Tevita Kete, PGR Officer Secretariat of the Pacific Community (SPC), Suva, Fiji Islands
This weblog documents the activities of Pacific Agricultural Genetic Resources Network (PAPGREN), along with other information on plant genetic resources (PGR) in the Pacific. The myriad varieties found within cultivated plants are fundamental to the present and future productivity of agriculture. PAPGREN, which is coordinated by the Land Resources Division of the Secretariat of the Pacific Community (SPC), helps Pacific countries and territories to conserve their crop genetic diversity sustainably, with technical assistance from the Bioversity International (BI) and support from NZAID and ACIAR. SPC also hosts the Centre of Pacific Crops and Trees (CEPaCT). The CEPaCT maintains regional in vitro collections of crops important to the Pacific and carries out research on tissue culture technology. The CEPaCT Adviser is Dr Mary Taylor (MaryT@spc.int), the CEPaCT Curator is Ms Valerie Tuia (ValerieT@spc.int). PAPGREN coordination and support IPGRI ACIAR NZAID CTA SPC PAPGREN CEPaCT Click on the thumbnail to see a map of the locations of Pacific genebanks. Click here to download a regional directory of genebanks in the Pacific, including information on their location, contact details and holdings. PAPGREN partners Mr William Wigmore Director of Research Ministry of Agriculture Department of Resources & Development P.O. Box 96 Rarotonga Cook Islands Tel: (682) 28711-29720 Fax: (682) 21881 Email: cimoa@oyster.net.ck Mr Adelino S. Lorens Chief Agriculture Pohnpei Office of Economic Affairs P.O. Box 1028 Kolonia Pohnpei 96941 Federated States of Micronesia Tel: (691) 3202400 Fax: (691) 3202127 Email: pniagriculture@mail.fm Dr Lois Englberger Island Food Community of Pohnpei Research Advisor P.O. Box 2299 Kolonia Pohnpei 96941 Federated States of Micronesia Email: nutrition@mail.fm Mr Apisai Ucuboi Director of Research Ministry of Agriculture, Fisheries & Forest Koronivia Research Station P.O. Box 77 Nausori Fiji Islands Tel: (679) 3477044 Fax: (679) 3477546-400262 Email: apisainu@yahoo.com Dr Maurice Wong Service du Developpement Rural B.P. 100 Papeete Tahiti 98713 French Polynesia Tel: (689) 42 81 44 Fax: (689) 42 08 31 Email: maurice.wong@rural.gov.pf Mr Tianeti Beenna Ioane Head, Research Section Division of Agriculture Ministry of Environment, Lands and Agricultural Development P.O. Box 267 Tarawa Kiribati Tel: (686) 28096-28108-28080 Fax: (686) 28121 Email : agriculture@tskl.net.ki; Beenna_ti@yahoo.com Mr Frederick Muller Secretary Ministry of Resources & Development P.O. Box 1727 Majuro 96960 Marshall Islands Tel: (692) 6253206 Fax: (692) 6257471 Email: rndsec@ntamar.net Mr Herman Francisco Director Bureau of Agriculture Ministry of Resources & Development P.O. Box 460 Koror 96940 Palau Tel: (680) 4881517 Fax: (680) 4881725 Email: bnrd@pnccwg.palaunet.com Ms Rosa Kambuou Principal Scientist PGR NARI Dry Lowlands Programme Laloki Agricultural Research Station P.O. Box 1828 Boroko National Capital District Papua New Guinea Tel: (675) 3235511 Fax: (675) 3234733 Email: kambuou@global.net.pg Ms Laisene Samuelu Principal Crop Development Officer Crops Division Ministry of Agriculture, Forests, Fisheries & Meteorology P.O. Box 1874 Apia Samoa Tel: (685) 23416-20605 Fax: (685) 20607-23996 Email: lsamuelu@lesamoa.net Mr Jimi Saelea Director of Research Department of Agriculture and Livestock P.O. Box G13 Honiara Guadalcanal Solomon Islands Tel: (677) 27987 Mr Tony Jansen Planting Materials Network Kastom Gaden Association Burns Creek, Honiara P.O. Box 742 Honiara Solomon Islands Tel: (677) 39551 Email: kastomgaden@solomon.com.sb Mr Finao Pole Head of Research Ministry of Agriculture & Forests P.O. Box 14 Nuku'alofa Tonga Tel: (676) 23038 Fax: (676) 24271 Email: thaangana@hotmail.com Mr Frazer Bule Lehi Head of Research Department of Agriculture & Rural Development Private Mail Bag 040 Port Vila Vanuatu Tel: (678) 22525 Fax: (678) 25265 Email: flehi@hotmail.com Other links Other CROP agencies Forum Secretariat University of the South Pacific SPREP Pacific biodiversity Biodiversity hotspots Breadfruit Institute Hawaiian native plants Intellectual property rights Nature Conservancy PBIF PestNet SIDS WWF South Pacific Program Other Pacific organizations Foundation of the Peoples of the South Pacific Micronesian Seminar Te Puna web directory Pacific news Cafe Pacific CocoNET Wireless Island Directory Pacific Islands News Pacific Islands Report Pacific Islands Travel Pacific Time South Pacific travel Time Pacific Interested in GIS? DIVA-GIS	Wednesday, May 07, 2008 Posted 3:06 PM by Tevita Fighting Fusarium in Asia-Pacific - ACIAR project update From : RISBAP The ACIAR-funded project, called “Mitigating the threat of banana Fusarium wilt: Understanding the agro-ecological distribution of pathogenic forms and developing disease management strategies”, continues to progress as it conducts survey and collection in Papua New Guinea, VCG analysis and molecular characterization, virulence-host resistance study, and establishment of on-farm demo-trials of disease management strategies. Survey and Collection in PNG. To date, survey and collection activities are being conducted in PNG. Survey teams included Jeff Daniells (Taxonomist), Pere Kokoa (Plant Pathologist), Birte Komolong (Plant Pathologist) and Janet Pafoa (Taxonomist). Survey was carried out between August to September 2007. A total of 27 samples from plants showing symptoms suspected to be of Fusarium wilt were collected from different provinces : Morobe Province/ Markham Valley, Kainantu, North Solomon Province/ Buka and ENB. The limited samples collected reflected the very low level of plants showing “Fusarium wilt symptoms”. No fusarium wilt symptoms were observed in ENB province. These samples were processed and then sent to QDPI, Australia for pathogen verification and VCG analyses. Other remaining provinces were to be surveyed late last year but was postponed to first quarter of 2008. These include Sandaun Province, Western Province and Manus Province, important provinces bordering Indonesia. These provinces were identified based on previous reports of suspicious Fusarium wilt symptoms in some areas. VCG analysis and Molecular Characterization. A total of 111 pure isolate-Foc samples from the Indonesian Tropical Fruits Research Institute (ITFRURI) were sent to QDPI for VCG and DNA characterization. To date, 80 of the 111 Indonesian isolates have been VCG tested. The rest have been analysed for volatile production and have produced mutant sectors. These samples are now ready for VCG testing. Based on the samples tested from different Indonesian provinces, majority of the positive results have been identified to belong to VCG 01213/16 (Tropical Race 4 – TR4). There are also positive results for other VCGs in Indonesian provinces and potentially new VCG record for Indonesia. The new records will be re-tested and re-confirmed, thus expanding the knowledge of the nature and distribution of Foc VCGs in Indonesia. A total of 27 samples from PNG for VCG testing were received by QDPI. No Foc pathogen was isolated from the 1st batch of 16 samples received from PNG. The 2nd batch of 11 samples are currently being tested. Virulence-host resistance study. A total of 25 varieties were selected for inoculation with various VCGs for virulence/host resistance test. Preliminary inoculation trial was done on 4 varieties namely: Ambon Kuning(AAA), Barangan (AAA), Kepok (ABB), and Rajasere (AAB). This is a screenhouse test that involves inoculating tissue culture plantlets, with selected VCGs. Then external as well as internal symptoms are quantified and recorded. A more complete inoculation studies on the 25 selected varieties are being established. On-farm disease management demo-trials of disease management strategies. A participatory planning workshop was conducted in the two sites on November 26 – 30, 2007. During the workshop, details of the methodology, field set up, and control practices to be evaluated were discussed and agreed upon. Planting materials were being prepared by ITFRURI. The establishment of the the demonstration plots was scheduled between January to February 2008. Survey and listing of existing private laboratories that produce banana tissue culture are being conducted in preparation for the implementation of the banana tissue cultured meriplant delivery system in Indonesia. (For more information contact Dr. A. Molina - Project Coordinator at a.molina@cgiar.org) * Comments: Post a Comment	Archive October 2002 November 2002 December 2002 January 2003 February 2003 March 2003 April 2003 May 2003 June 2003 July 2003 August 2003 September 2003 October 2003 November 2003 December 2003 January 2004 February 2004 March 2004 April 2004 May 2004 June 2004 July 2004 August 2004 September 2004 October 2004 November 2004 December 2004 January 2005 February 2005 March 2005 April 2005 May 2005 June 2005 July 2005 August 2005 September 2005 October 2005 November 2005 December 2005 January 2006 February 2006 March 2006 April 2006 May 2006 June 2006 July 2006 August 2006 September 2006 October 2006 November 2006 December 2006 January 2007 February 2007 March 2007 April 2007 May 2007 June 2007 July 2007 August 2007 September 2007 October 2007 November 2007 December 2007 January 2008 February 2008 March 2008 April 2008 May 2008 June 2008 July 2008 August 2008 September 2008 October 2008 November 2008 December 2008 January 2009 February 2009 March 2009 April 2009 May 2009 June 2009 July 2009 August 2009 September 2009 October 2009 November 2009 January 2010 RSS Feed Alternative feed Contact Tevita Something new: Agrobiodiversity Weblog: For discussions of conservation and sustainable use of the genetic resources of crops, livestock and their wild relatives. PestNet: For on-line information, advice and pest identification for the Pacific and beyond. Contact: Grahame Jackson. Pacific Mapper: For on-line mapping of point data over satellite images of the Pacific provided by Google Maps. DIVA-GIS: For free, easy-to-use software for the spatial analysis of biodiversity data.